Research and Development Plan
Research and Development Plan
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Overview of Transgenic Plant Development
Assemble Constructs
Transform Plant Callus Tissue
Select Fluorescent Calli
Regenerate Plants
Transformed Plant Callus Tissue without Antibiotic Resistance Genes
Lack of Resistance Genes Prevents Unintended Environmental Transfer
Lack of Resistance Genes also allows Direct Marketability and is Compliant with United States Transgenic Guidelines
Removing resistance genes enables direct-to-consumer use, streamlines regulatory approval, and enhances marketability for ornamental and research applications.
Transgenic Selection using only Fluorescence as a Selectable Marker
Timeline for Project
Weeks 1–4: Design and assemble gene constructs for fluorescent reporters.
Weeks 5–8: Transform aquatic plant callus tissue using Agrobacterium.
Weeks 9–15: Select bright and analyte-responsive calli using fluorescence.
Weeks 15–36: Quantify analyte-induced and constitutive fluorescence in regenerated plantlets.
Weeks 1–78: Continuously evolve and optimize fluorescent proteins for enhanced brightness and IP freedom.
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